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It is known that dissolved organic compounds in OSPW are responsible for most toxic effects, but knowledge of the specific mechanism(s) of toxicity, is limited.Using bioassay-based effects-directed analysis, the dissolved organic fraction of OSPW has previously been fractionated, ultimately producing refined samples of dissolved organic chemicals in OSPW, each with distinct chemical profiles.Constructed from MD simulations, MSMs can elucidate the conformational dynamics of AGO at biologically relevant timescales.Protein-RNA docking can then efficiently identify the AGO conformations that are geometrically accessible to mi RNA.The understanding of abscission is of great importance to control seed and fruit production and to improve breeding and harvesting practices.Thus, advances made on model plants and crops are of major importance since they may provide potential candidate genes for further biotechnological applications.Here, we review the current knowledge of the physiological, genetic and genomic aspects related to abscission including the most recently disclosed putative regulators that appear to be implicated in the development and/or activation of the AZs.► Abscission is introduced as a production-limiting factor for crop species.

► Current knowledge regarding the molecular factors that take part in the ontogeny and activation of the AZ is discussed.Using the K-12 strain MG1655 gene reporter live cell array, the present study investigated relationships between toxic potencies of each fraction, expression of genes and characterization of chemicals in each of five acutely toxic and one non-toxic extract of OSPW derived by use of effects-directed analysis.Effects on expressions of genes related to response to oxidative stress, protein stress and DNA damage were indicative of exposure to acutely toxic extracts of OSPW.Little is known, however, about how γ-Pcdh function is regulated by other factors.Here I show that PKC phosphorylates a serine residue situated within the shared γ-Pcdh C-terminus; PKC phosphorylation disrupts the γ-Pcdhs’ inhibition of FAK.




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